Role of vanilloid receptors in thermal hyperalgesia induced by intraplantar endothelin-1 administration.
- Author:
Wen-Jin JI
1
;
Jie-Xian LIANG
;
Guo-Dong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dose-Response Relationship, Drug; Endothelin-1; administration & dosage; toxicity; Genotype; Hot Temperature; Hyperalgesia; chemically induced; physiopathology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Pain Measurement; methods; TRPV Cation Channels; genetics; physiology
- From: Journal of Southern Medical University 2007;27(1):101-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the role of vanilloid receptor (VR) in thermal hyperalgesia induced by intraplantar endothelin-1 (ET-1) injection.
METHODSVR gene-knockout mice (KO group) and wild type C57BL/6J mice (WT group) in 3 subgroups were subjected to intraplantar administration of ET-1 at the doses of 3, 10, 30 and 100 pmol (dissolved in 10 microl of PBS, pH 7.4, n=6 in each group), respectively. The latency time of paw withdrawal (PWT) from heat irradiation stimulation was recorded before injection and 15, 30, 45 and 60 min after injection.
RESULTSET-1 induced thermal hyperalgesia in both groups. The mice in WT group showed a more sharply shortened PWT than those in KO group. ET-1 decreased PWT as the dose administered increased in WT group, which was different from the responses of the KO mice. At the dose of 100 pmol of ET-1, no further decrement of latency time was observed in WT group, whereas such response occurred at 30 pmol in KO group.
CONCLUSIONIntraplantar injection of ET-1 induces thermal hyperalgesia mediated partially by VR.
