Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues.
- Author:
Shuang WANG
1
;
Li-Chun LU
;
Wei-Yi FANG
;
Kai-Tai YAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; pathology; E2F1 Transcription Factor; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Nasopharyngeal Neoplasms; genetics; pathology; Nuclear Proteins; genetics; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors; genetics; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2007;27(2):146-149
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.
METHODSThe total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.
RESULTSAmong the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.
CONCLUSIONThese differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.
