Exploration of the alternative splicing variants of rat phospholipase C-gamma 1 pre-mRNA.
- Author:
Zhong-Ying LIU
1
;
Shen-Qiu LUO
;
Yong-Zhong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Alternative Splicing; Animals; Base Sequence; Molecular Sequence Data; Phospholipase C gamma; genetics; RNA Precursors; genetics; RNA Splice Sites; genetics; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Journal of Southern Medical University 2007;27(2):191-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of phospholipase C-gamma 1 (PLC-gamma1) alternative splicing variants in rats.
METHODSAccording to the sequence of human PLCG1 splicing variant, specific primers for rat PLC-gamma1 were designed and synthesized. The rat RNA was reverse transcribed into cDNA, which was amplified using the specific primers, and the PCR products were sequenced and analyzed using BLAST and bioinformatics methods. Totally 21 rat tissue samples were examined, including the heart, liver, lung, kidney, eyeball, and brain obtained in 3 different embryonic stages, 7 different early postnatal stages, and in adulthood.
RESULTSThe result did not show that rat PLC-gamma1 had the same splicing variant (PLC-gamma1a, NM_002660) as human does.
CONCLUSIONSThe same splicing variant of PLC-gamma1 detectable in human may not exist in rats, and the pre-mRNA may undergo splicing resulting predominantly in PLC-gamma1b mRNA. Very likely, the alternative splicing site of rat PLC-gamma1 is not identical to that of human.
