Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1.
- Author:
Li-Hua ZHU
1
;
Tian-Ji ZHOU
;
Guo-You SHI
;
Guang-Ling ZHANG
;
Shu-Ying LI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Transformed; Cell Transformation, Viral; Epithelial Cells; cytology; metabolism; virology; Herpesvirus 4, Human; Humans; Immunohistochemistry; Proto-Oncogene Proteins c-bcl-2; biosynthesis; Stomach; cytology
- From: Journal of Southern Medical University 2007;27(2):195-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.
METHODSAkata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.
RESULTSBcl-2 protein expression was detected in EBV-infected cells but not in the control cells.
CONCLUSIONEBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.
