Matrine-induced gamma-globin synthesis in K562 cells.
- Author:
Cui-Mei ZHANG
1
;
Zhi-Chun FENG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Blotting, Western; Fetal Hemoglobin; biosynthesis; Globins; biosynthesis; Humans; K562 Cells; Quinolizines; pharmacology
- From: Journal of Southern Medical University 2007;27(2):215-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of matrine on gamma-globin synthesis in K562 cells in vitro.
METHODSBenzidine staining was used to study the dose- and time-dependent effects of matrine on hemoglobin synthesis in K562 cells, and Western blotting was performed to determine the level of hemoglobin F(alpha(2)gamma(2)).
RESULTSBenzidine staining showed that K562 cells treated with matrine of 0.1 mg/ml had a positivity rate for benzidine (BZ%) of 15.67% at 96 h after the treatment, and Western blotting indicated increased synthesis of hemoglobin F.
CONCLUSIONMatrine can induce gamma-globin synthesis and increase hemoglobin F level in K562 cells, the effect of which resembles that of sodium butyrate.
