Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs.
- Author:
Ming-xin PAN
1
;
Fan-dong KONG
;
Zhi-feng WU
;
Yi ZHANG
;
Hai-lan WANG
;
Ai-hui LI
;
Jin-ming XIE
;
Yi GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Graft Survival; physiology; HSP70 Heat-Shock Proteins; metabolism; Ischemic Preconditioning; Liver; blood supply; metabolism; physiopathology; Liver Transplantation; Male; Random Allocation; Swine; Swine, Miniature
- From: Journal of Southern Medical University 2007;27(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.
METHODSTwenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy.
RESULTSIschemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas ischemia-reperfusion caused a significant increase of serum transaminases level (P<0.01) with declined HSP70 expression and obvious microstructural changes of the liver tissue.
CONCLUSIONIschemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.
