Removal of the template molecules from vinblastine-imprinted polymer.
- Author:
Jian-yong FENG
1
;
Quan-hong ZHU
;
Jia-bo LUO
Author Information
- Publication Type:Journal Article
- MeSH: Molecular Imprinting; methods; Plants, Medicinal; chemistry; Polymers; chemistry; Reproducibility of Results; Solvents; chemistry; Vinblastine; isolation & purification
- From: Journal of Southern Medical University 2007;27(3):268-271
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the optimal condition for complete removal of the template molecules from vinblastine (VLB)-imprinted polymer.
METHODThe prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions. The contents and recoveries of VLB in the effluents were determined.
RESULTSPolymer extraction with methanol-glacial acetic acid (9:1, V/V) resulted in VLB recovery of 91.73%, but template bleeding was observed because of incomplete VLB removal. Using methanol-glacial acetic acid (6:4, V/V) as the extraction solvent, the recovery of VLB reached 98.03% with less solvents and extract times. The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid (6:4, V/V).
CONCLUSIONMethanol-glacial acetic acid (6:4, V/V) is an ideal extract solvent for complete template molecule removal from the polymers, and the processed polymers possess stable capacity of specific recognition and selectivity to the template.
