Preparation of cisplatin-impregnated coral hydroxyapatite drug delivery system.
- Author:
Jin-cheng YANG
1
;
Qing-shui YIN
;
Jun LIN
;
Hua-yang HUANG
;
Huan-wen DING
;
Yu ZHANG
;
Jian LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anthozoa; chemistry; Antineoplastic Agents; administration & dosage; chemistry; pharmacology; Cell Line, Tumor; Cell Survival; drug effects; Cisplatin; administration & dosage; chemistry; pharmacology; Drug Compounding; methods; Drug Delivery Systems; Durapatite; chemistry; Humans; Microscopy, Electron; Time Factors
- From: Journal of Southern Medical University 2007;27(3):283-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a cisplatin-impregnated coral-derived hydroxyapatite (CCHA) drug delivery system (DDS), and evaluate its inhibitory effect on human osteosarcoma cells U-2 OS, human breast cancer and prostatic carcinoma cells PC-3 in vitro.
METHODSThe coral-derived hydroxyapatite (CHA) was manufactured by hydrothermal exchange and impregnated with cisplatin by vacuum freeze-drying techniques. The leaching solutions of this DDS was collected at different intervals in a course of 8 weeks and their inhibitory effect on the cells was tested in vitro by MTT assay.
RESULTSElectron microscope showed that cisplatin was distributed homogeneously in the pores of CHA. The inhibition rates of the leaching solution on all the tumor cells exceeded 50% except for PC-3 cells, whose inhibition rate was 29.92% when treated with the solution collected at the eighth week.
CONCLUSIONCCHA allows sustained drug release and maintains excellent inhibitory effect on human bone tumor cells within 8 weeks in vitro.
