Fluorescence in-situ hybridization as a diagnostic tool for cutaneous melanoma.

Jing SU; Jianying Liu; Jie Zheng; Jiangfeng You; Xiaolong MA; Yan Zhang; Jing Zhang; Songlin Liao

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Fluorescence in-situ hybridization as a diagnostic tool for cutaneous melanoma.

Author: Jing SU ¹ ; Jianying LIU ² ; Jie ZHENG ¹ ; Jiangfeng YOU ¹ ; Xiaolong MA ¹ ; Yan ZHANG ¹ ; Jing ZHANG ¹ ; Songlin LIAO ¹
Author Information

1. Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University,Beijing 100191, China.
2. Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University,Beijing 100191, China. E-mail: liujianying@bjmu.edu.cn.
Publication Type:Journal Article
MeSH: Chromosome Aberrations; Cyclin D1; genetics; Diagnosis, Differential; Humans; In Situ Hybridization, Fluorescence; Melanoma; diagnosis; genetics; Nevus; diagnosis; Sensitivity and Specificity; Skin Neoplasms; diagnosis; genetics
From: Chinese Journal of Pathology 2015;44(1):37-41
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the utility of fluorescence in situ hybridization as a diagnostic tool for cutaneous melanoma.
METHODSTwenty cutaneous melanomas and 20 cutaneous nevi from pathology files were selected and analyzed by Vysis melanoma FISH probe kit targeting 3 loci on chromosome 6 (MYB, CEP6 and RREB1) and 1 locus on 11q (CCND1) and data were interpreted based on the Abbott criteria provided by the kit.
RESULTSInformative FISH results were obtained in 16 melanomas and 18 nevi. Chromosomal aberrations were detected in 12 of the 16 melanomas and only 1 of 18 nevi.
CONCLUSIONFISH is a useful diagnostic tool and able to distinguish cutaneous nevus from melanoma with good sensitivity and specificity.