Protection effect of amentoflavone in Selaginella tamariscina against TNF-alpha-induced vascular injury of endothelial cells.
- Author:
Xiao-ke ZHENG
1
;
Cai-xia LIU
1
;
Ying-ying ZHAI
1
;
Ling-ling LI
1
;
Xiao-lan WANG
1
;
Wei-sheng FENG
1
Author Information
1. Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.
- Publication Type:Journal Article
- MeSH:
Biflavonoids;
isolation & purification;
pharmacology;
Cell Cycle;
drug effects;
Cell Survival;
drug effects;
E-Selectin;
metabolism;
Endothelin-1;
metabolism;
Human Umbilical Vein Endothelial Cells;
Humans;
I-kappa B Proteins;
metabolism;
Interleukin-6;
metabolism;
Interleukin-8;
metabolism;
Malondialdehyde;
metabolism;
NF-KappaB Inhibitor alpha;
Nitric Oxide;
metabolism;
Plants, Medicinal;
chemistry;
Protective Agents;
isolation & purification;
pharmacology;
Selaginellaceae;
chemistry;
Superoxide Dismutase;
metabolism;
Transcription Factor RelA;
metabolism;
Tumor Necrosis Factor-alpha;
adverse effects;
Vascular Cell Adhesion Molecule-1;
metabolism
- From:
Acta Pharmaceutica Sinica
2013;48(9):1503-1509
- CountryChina
- Language:Chinese
-
Abstract:
This study is to observe the protection effect of amentoflavone (AMT) in Selaginella tamariscina against TNF-alpha-induced vascular inflammation injury of endothelial cells. On the basis of TNF-alpha induced human umbilical vein endothelial cell, observe the influence of AMT on endothelial active factor, the contents of SOD and MDA, the protein expression of vascular endothelial adhesion molecules and inflammatory factor; study the effect of its common related signal pathways such as NF-kappaB; research the effect of AMT against TNF-a induced human umbilical vein endothelial cell injury by means of MTT, ELISA, Western blotting and the cell immunofluorescence. The results showed that AMT could increase the content of NO and decrease the levels of VCAM-1, E-selectin, IL-6, IL-8 and ET-1; enhance the activity of SOD, reduce the content of MDA; downregulate the protein expressions of VCAM-1, E-selectin, NF-kappaBp65 and up-regulate IkappaBalpha, attenuate the NF-kappaBp65 transfer to cell nucleus. AMT has the effect of protect vascular endothelial and maybe via the signal pathway of NF-kappaB to down-regulate the inflammation factor and oxidative damage factor of downstream.
