Between Hengshanhuangqi and Chuanhuangqi based on metabolomics and ITS2 sequences.
- Author:
Pan HE
1
;
Zhen-Yu LI
1
;
Sheng-Ci FAN
2
;
Fu-Sheng ZHANG
1
;
Xue-Mei QIN
1
;
Guo-Jun DU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Taiyuan 030006, China.
2. Shanxi Zhendong Genuine Medicinal Materials Develepment Co., Ltd., Changzhi 047100, China.
- Publication Type:Journal Article
- MeSH:
Astragalus membranaceus;
chemistry;
classification;
genetics;
Base Sequence;
DNA, Plant;
genetics;
DNA, Ribosomal Spacer;
genetics;
Glucosides;
analysis;
Isoflavones;
analysis;
Metabolomics;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry;
classification;
genetics;
Principal Component Analysis;
Saponins;
analysis;
Sequence Analysis, DNA;
Species Specificity;
Triterpenes;
analysis
- From:
Acta Pharmaceutica Sinica
2013;48(10):1595-1601
- CountryChina
- Language:Chinese
-
Abstract:
To compare the differences between Hengshanhuangqi (HH) and Chuanhuangqi (CH) at molecular level, 1H NMR based plant metabolomics approach was used to reveal the chemical difference between HH and CH. Then, the contents of astragaloside IV and calycosin-7-O-beta-D-glucoside, the marker compounds specified in China Pharmacopoeia, were determined. In addition, the ITS2 fragments of HH and CH were sequenced. Twenty-three metabolites were identified in the 1H NMR spectrum, and the principal component analysis showed CH and HH could be separated clearly. HH contained more aspartic acid, GABA, citric acid, astragaloside IV and calycosin-7-O-beta-D-glucoside, while CH contained more threonine, alanine, acetic acid, choline, arginine, fructose and sucrose. And the astragaloside IV is almost undetectable in CH. In addition, the ITS2 fragment sequences of HH and CH were different at eight bases. Thus, the HH and CH showed significant differences chemically and genetically.
