Study on different factors affecting the bionic enzymatic hydrolysis of icariin.
- Author:
Xia GAO
1
;
Yan CHEN
2
;
Ying WANG
2
;
Wen-jie SUN
1
;
Xiao-bin JIA
2
Author Information
1. Affiliated Hospital on Intergration of Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China.
2. Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cellulase;
chemistry;
Flavonoids;
biosynthesis;
metabolism;
Hydrolases;
chemistry;
isolation & purification;
Hydrolysis;
Pancreatin;
chemistry;
Snails;
enzymology;
Surface-Active Agents;
chemistry;
beta-Glucosidase;
chemistry
- From:
Acta Pharmaceutica Sinica
2013;48(11):1716-1721
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to observe different factors which affected the bionic enzymatic hydrolysis of icariin into baohuoside I and to optimize the reaction conditions in order to provide research foundation for building a novel bionic enzymolysis drug delivery system. To simulate the environment in vivo, 37 degrees C was set as the temperature and artificial intestinal juice and gastric juice were selected as the buffer solutions. Taking the conversion of baohuoside I as index, the effects of the kinds of enzyme, enzyme activity, substrate concentration, reaction time, pancreatin in artificial intestinal juice and surfactant on the conversion of baohuoside I were investigated. The results showed that cellulase, beta-glucosidase and snailase were all inactive in artificial gastric juice and no baohuoside I generated. Pancreatin in artificial intestinal juice couldn't significantly influence the activity of beta-glucosidase or snailase (P > 0.05), but noticeably decrease the activity of cellulase (P < 0.05). In artificial intestinal juice, the conversion of baohuoside I was highest by using beta-glucosidase, and the optimum reaction conditions were determined as follows: enzyme activity 10 U x mL(-1), substrate concentration 1 mg x mL(-1), 3 g x L(-1) rhamnolipid and reaction time 3 h. Under this condition, the conversion of baohuoside I was 99.8%.
