Primary co-culture of cortical neurons and astrocytes of new-born SD rats.
- Author:
Cheng-na WANG
1
;
Li LIN
1
;
Zhen-fang DUAN
1
;
Fei ZHONG
1
;
Dai-ying ZUO
1
;
Ying-liang WU
1
Author Information
1. School of Life Science and Biopharmaceutic, Shenyang Pharmaceutical University, Shenyang 110016, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Astrocytes;
cytology;
Cells, Cultured;
Cerebral Cortex;
cytology;
Coculture Techniques;
methods;
Female;
Male;
Neurons;
cytology;
Primary Cell Culture;
methods;
Rats;
Rats, Sprague-Dawley
- From:
Acta Pharmaceutica Sinica
2013;48(11):1729-1732
- CountryChina
- Language:Chinese
-
Abstract:
This study is to establish a simple and practical co-culture method of cortical neurons and astrocytes of rats. The cortex of the new-born SD rats was digested by 0.125% pancreatic enzyme, and the differential adherence was applied to obtain the mixed cell suspension of neurons and astrocytes. A low concentration of cytarabine was used to inhibit the astrocytes in a moderate way to get neuronal and astrocyte co-culture. The morphological characteristics of the cells in different times were observed under the inverted microscope. The cells began to adhere the wall 2 h after the inoculation. Neurons and astrocytes grew in a good condition under the inverted microscope 9 days after the inoculation. The results of the immunofluorescence staining and Rosenfeld's staining indicated that the co-culture of neurons and astrocytes was successful and the ratio of neurons and astrocytes was close to 1:1. A new neurons and astrocytes co-culture method, which is simple and convenient, was successfully established. It will be an efficient method for the related researches about neuronal and astrocyte co-culture in vitro.
