Fragmentation behaviors of taxanes drugs using electrospray ionization with quadrupole time-of-flight mass spectrometry.
- Author:
Wen-Xiao MA
;
Hao WANG
;
Ting-Ting WANG
;
Yan YANG
;
Jing-Kai GU
- Publication Type:Journal Article
- MeSH:
Paclitaxel;
chemistry;
Peptide Fragments;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
methods;
Taxoids;
chemistry
- From:
Acta Pharmaceutica Sinica
2013;48(12):1812-1816
- CountryChina
- Language:Chinese
-
Abstract:
The fragmentation pathways of two taxanes drugs have been studied in positive ion mode by Q-TOF with the advantages of high mass accuracy and high resolution analysis. The [M+H] + ions were observed by ESI-MS, from which the molecular weights were obtained. Using the protonated pseudo-molecular ions [M+H]+ as internal reference compounds, the accurate mass and element composition of the fragment ions were determined. The collision induced dissociation (CID) data of the [M+H] ions provided fragmentation pathways of related compounds. Results showed that the major cleavage pathways of paclitaxel and docetaxel were the same that the cleavage of C-O bond between the side chain and taxol skeleton easily occurred, then stripping of the functional groups on the parent ring. Some common fragments were formed, such as m/z 105.033 7, 291.137 3, 309.148 5, 327.159 7, 387.181 2 and 509.217 4, which would provide a basis for future qualitative and quantitative analysis of taxanes in vitro and in vivo.
