The synergistic effect of lidamycin and rituximab on human B cell lymphoma.
- Author:
Yi-Ran SUN
;
Sheng-Hua ZHANG
;
Rong-Guang SHAO
;
Hong-Wei HE
- Publication Type:Journal Article
- MeSH:
Aminoglycosides;
pharmacology;
Animals;
Antibiotics, Antineoplastic;
pharmacology;
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Caspase 7;
metabolism;
Caspase 9;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drug Synergism;
Enediynes;
pharmacology;
Humans;
Inhibitor of Apoptosis Proteins;
metabolism;
Lymphoma, B-Cell;
metabolism;
pathology;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Neoplasm Metastasis;
Neoplasm Transplantation;
Poly(ADP-ribose) Polymerases;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Random Allocation;
Rituximab;
pharmacology
- From:
Acta Pharmaceutica Sinica
2014;49(2):198-203
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the synergistic effect of lidamycin (LDM) and rituximab on human B cell lymphoma Ramos cells. Cell proliferation was measured using MTS assay, cell apoptosis was analyzed by Annexin V-FITC/PI assay, the expression of apoptosis related proteins was analyzed by Western blotting, and the in vivo lymphoma inhibition was verified using BALB/c mice inoculated via tail vein using Ramos cells which stably expressed pEGFP-N1 plasmid. The results showed that, after the pretreatment with rituximab for 48 h, rituximab and LDM showed significantly synergistic effects on cell proliferation. Cells in combined treatment group had a higher apoptosis rate than that in LDM treatment group. Compared with the LDM treatment group, the expression of apoptosis-related proteins such as Cleaved caspase-3, Cleaved caspase-7, Cleaved caspase-9 and Cleaved PARP in combined treatment groups increased, and expression of cIAP-2 and Bcl-2 decreased. The result of in vivo experiment showed that, in the combined treatment group, the survival time of BALB/c mice was significantly longer than the mice in control group and LDM treatment group, and the degree of tumor accumulation and metastasis to lymph nodes and spleen was lower.
