Quantitative determination of bosentan by proton nuclear magnetic resonance with internal standard method.
- Author:
Cai-Yu ZHANG
;
Na ZHANG
;
Lan HE
- Publication Type:Journal Article
- MeSH:
Calibration;
Magnetic Resonance Spectroscopy;
methods;
Molecular Structure;
Protons;
Quality Control;
Sulfonamides;
chemistry
- From:
Acta Pharmaceutica Sinica
2014;49(2):249-251
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to establish a quantitative nuclear magnetic resonance (QNMR) method for the determination of the absolute content of bosentan. Proton nuclear magnetic resonance spectroscopy [1H NMR] spectra were obtained in CDCl3 with the internal standard dimethyl terephthalate and zg30 pulse sequence by using a Bruker AVANCE II 400 spectrometer. The content of bosentan is determined with QNMR in comparison with the result obtained by mass balance method. The result is 96.25% by QNMR and 96.54% by mass balance method. A rapid and accurate QNMR method has been established for the quantitative determination of the absolute content of bosentan. The study provides a new way for the quality control and calibration of a new reference standard material, it could be the complementary with the mass balance method for the assay of standard reference.
