Enhancers on the transmembrane transport of chlorogenic acid.
- Author:
Jing REN
;
Sheng-Qi DENG
;
Xue-Hua JIANG
;
Ling-Ling WANG
;
Yu XIAO
- Publication Type:Journal Article
- MeSH:
Absorption;
Acrylic Resins;
pharmacology;
Caco-2 Cells;
Cell Membrane Permeability;
drug effects;
Chlorogenic Acid;
pharmacokinetics;
Humans;
Sodium Dodecyl Sulfate;
pharmacology;
Taurocholic Acid;
pharmacology
- From:
Acta Pharmaceutica Sinica
2014;49(2):252-255
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the influence of the difference enhancers on the transport mechanism of chlorogenic acid (CGA) across Caco-2 cells model, a RP-HPLC method was adopted to detect the concentrations of CGA. At the concentrations of 20 to 80 microg x mL(-1), the difference of absorption rate constants (K(a)) was not statistically significant. At the concentrations of 40 and 20 microg x mL(-1), the ratios of apparent permeability coefficients (P(app)) of the apical to basolateral and the basolateral to apical were 1.14 and 1.18, respectively. With the effect of enhancers K(a) and P(app) increased, the absorption half-life (T1/2) decreased. CGA passed through the Caco-2 cell membrane mainly by passive transport. It showed that monocarboxylic acid transporter (MCT) could be involved in the across membrane transport process of CGA. Borneol had no effect on the cell membrane transport processes. The order of increasing absorption of CGA caused by the enhancers was sodium lauryl sulphate > sodium taurocholate > carbomer.
