Repeated injection of mitoxantrone containing thermosensitive liposomes in rat induced ABC phenomenon.
- Author:
Wei TIAN
;
Lan ZHANG
;
Na WEI
;
Chan LI
;
Bei-Bei NI
;
Xi ZHAO
;
Chun-Lei LI
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents;
administration & dosage;
blood;
pharmacokinetics;
Chromatography, High Pressure Liquid;
Immunoglobulin M;
blood;
Liposomes;
administration & dosage;
blood;
pharmacokinetics;
Male;
Metabolic Clearance Rate;
Mitoxantrone;
administration & dosage;
blood;
pharmacokinetics;
Polyethylene Glycols;
administration & dosage;
chemistry;
pharmacokinetics;
Rats;
Rats, Wistar;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2014;49(2):256-259
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.
