Identification of radix et rhizoma clematidis and its adulterants using DNA barcoding.
- Author:
Shan-Shan FENG
;
Si-Hao ZHENG
;
Ya-Kang LI
;
Lin-Fang HUANG
- Publication Type:Journal Article
- MeSH:
DNA Barcoding, Taxonomic;
methods;
DNA, Plant;
genetics;
Drug Contamination;
Nucleic Acid Amplification Techniques;
methods;
Plant Roots;
genetics;
Plants, Medicinal;
classification;
genetics;
Ranunculaceae;
classification;
genetics;
Rhizome;
genetics;
Species Specificity
- From:
Acta Pharmaceutica Sinica
2014;49(2):260-266
- CountryChina
- Language:Chinese
-
Abstract:
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
