High performance preparation and structural confirmation of lignans from Schisandrae chinensis fructus by using HSCCC combined with ESI-MSn method.
- Author:
Xiao-Li YU
;
Zi-feng PI
;
Xiu-Li HU
;
Feng-Rui SONG
;
Zhi-Qiang LIU
- Publication Type:Journal Article
- MeSH:
Countercurrent Distribution;
Cyclooctanes;
chemistry;
isolation & purification;
Dioxoles;
chemistry;
isolation & purification;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Fruit;
chemistry;
Lignans;
chemistry;
isolation & purification;
Molecular Structure;
Plants, Medicinal;
chemistry;
Polycyclic Compounds;
chemistry;
isolation & purification;
Schisandra;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2014;49(1):78-82
- CountryChina
- Language:Chinese
-
Abstract:
High-speed counter-current chromatography (HSCCC) was used to high performance separate and prepare lignans from Schisandrae chinensis fructus. The solvent system is composed of n-hexane-ethyl acetate-methanol-water (9 : 1 : 5 : 5) and n-hexane-ethyl acetate-methanol-water (9 : 1 : 9 : 5), speed is at 900 r.min-1, and flow rate is at 2.0 mL.min-1. Five fractions from Schisandrae chinensis fructus extract were separated and prepared with one HSCCC process. They were identified as schisandrin, gomisin J, schisandrol B, schisantherin A and deoxyschizandrin by electrospray ionization-multiple tandem mass spectrometry (ESI-MSn), respectively. Their contents were obtained in 98.74%, 94.32%, 99.53%, 94.23% and 98.68% by ultra high performance liquid chromatography (UPLC), separately. The rapid and simple method can be applied for the preparation of lignans from Schisandrae chinensis fructus.
