Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus.
- Author:
Zheng NI
1
;
Wei WEI
;
Guang-Qing LIU
;
Liu CHEN
;
Bin YU
;
Tao YUN
;
Jiong-Gang HUA
;
Shuang-Mao LI
Author Information
1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Caliciviridae Infections;
genetics;
physiopathology;
veterinary;
virology;
Caspase 3;
metabolism;
Cell Line;
Cell Nucleus;
genetics;
virology;
DNA Fragmentation;
Hemorrhagic Disease Virus, Rabbit;
physiology;
Rabbits
- From:
Chinese Journal of Virology
2009;25(4):316-317
- CountryChina
- Language:Chinese
-
Abstract:
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
