Effect of simian vacuolating virus 40 on development and differentiation of dendritic cells from Rhesus macaque.
- Author:
Chang-Yong GE
1
;
Hong-Jun LI
;
Zhan-Long HE
;
Guang-Ming ZHANG
;
Mao-Sheng SUN
;
Nan-Ping WU
Author Information
1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD;
metabolism;
Antigens, CD1;
metabolism;
B7-2 Antigen;
metabolism;
Cell Differentiation;
Cells, Cultured;
Dendritic Cells;
cytology;
immunology;
virology;
Flow Cytometry;
HLA-DR Antigens;
metabolism;
Immunoglobulins;
metabolism;
Macaca mulatta;
Membrane Glycoproteins;
metabolism;
Polyomavirus Infections;
physiopathology;
Simian virus 40;
physiology
- From:
Chinese Journal of Virology
2009;25(5):344-348
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of simian vacuolating virus 40 (SV40) on development and differentiation of dendritic cells (DC) from rhesus macaque, the peripheral blood-derived dendritic cells from rhesus monkey were pulsed with inactivated SV40 and infective SV40, respectively at the 5th day post DC cultivation. Expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface at the 7th, 9th day post DC cultivation were analyzed by flow cytometry (FCM). The results showed that expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface in the inactivated SV40-pulsed experimental group were higher than those in the infective SV40-pulsed experimental group (P < 0.05). These cell surface molecules represented characteristic development and differentiation phase of DC. Down-regulation of expressions of these cell surface molecules indicated that infective SV40 might hamper differentiation and maturation of dendritic cells from rhesus monkey.
