Biological characteristics of a chimeric rabies virus expressing canine parvovirus VP2 protein.
- Author:
Xue-Feng NIU
1
;
Xiao-Hui LIU
;
Zhao-Jin SUN
;
He-He SHI
;
Jing CHEN
;
Bido JIANG
;
Jing-Chen SUN
;
Xiao-Feng GUO
Author Information
1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Capsid Proteins;
genetics;
immunology;
Cell Line;
Cricetinae;
Female;
Mice;
Parvovirus, Canine;
genetics;
immunology;
Rabies;
immunology;
virology;
Rabies virus;
genetics;
immunology;
Reverse Transcriptase Polymerase Chain Reaction;
Viral Vaccines;
genetics;
immunology
- From:
Chinese Journal of Virology
2009;25(5):362-367
- CountryChina
- Language:Chinese
-
Abstract:
To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury (VP2) grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells. Experiments on the mice immunized with the chimeric virus HEP-Flury (VP2) demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.
