Molecular identification of human enterovirus 73 in Yunnan Province, the People's Republic of China.
- Author:
Jing-Jing TANG
1
;
Zheng-Rong DING
;
Bing-Jun TIAN
;
Zhi-Xian ZHAO
;
Jie ZHANG
;
Li-Qun LI
;
Li-Fang HE
Author Information
1. Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China. tangjingjing090101@126.com
- Publication Type:Journal Article
- MeSH:
Cell Line;
Child;
China;
DNA Primers;
genetics;
Enterovirus;
classification;
genetics;
isolation & purification;
Enterovirus Infections;
virology;
Female;
Humans;
Male;
Molecular Sequence Data;
Phylogeny
- From:
Chinese Journal of Virology
2009;25(6):407-409
- CountryChina
- Language:Chinese
-
Abstract:
Molecular typing was conducted according to the reported methods for 2 enteroviruses that were isolated from healthy children in the border areas of Yunnan Province with Myanmar. RT-PCR and sequencing were performed with 292/222 primers according to the Oberste's methods. The resulting sequences were blasted against the Genbank database and compared with all available enterovirus database. Analysis of homology at nucleotide and amino acid level identically suggested that the two enteroviruses are human enterovirus 73.
