Genotype distribution of enterovirus A species isolated in Shandong Province, China.
- Author:
Ze-Xin TAO
1
;
Yan LI
;
Hai-Yan WANG
;
Li-Zhi SONG
;
Gui-Fang LIU
;
Yao LIU
;
Xiao-Juan LIN
;
Lei FENG
;
He YANG
;
Qing-Ying FAN
;
Ai-Qiang XU
Author Information
1. Shandong Center for Disease Control and Prevention, Jinan 250014, China. zexin.tao@163.com
- Publication Type:Journal Article
- MeSH:
Cell Line;
China;
Enterovirus A, Human;
classification;
genetics;
isolation & purification;
Enterovirus Infections;
virology;
Feces;
virology;
Genotype;
Hand, Foot and Mouth Disease;
virology;
Humans;
Molecular Sequence Data;
Paraplegia;
virology;
Phylogeny
- From:
Chinese Journal of Virology
2009;25(6):410-414
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the genotypes and molecular evolution of human enterovirus (HEV) A species in Shandong Province, Stool samples were collected from AFP and HFMD patients in Shandong Province and virus isolation was performed. Reverse Transcription-Polymerase Chain Reactions (RT-PCR) specific for EV71 and CVA16 were performed with the virus isolates from HFMD patients. Positive isolates were selected for entire VP1 coding gene amplification and sequencing. Isolates with negative PCR results and isolates from AFP patients were selected for entire VP1 coding gene amplification and sequencing using primers specific for HEV A species. Phylogenetic tree was constructed among these VP1 nucleotide sequences and of other strains. Altogether 293 strains classified into 8 genotypes were isolated. The homologous comparison and phylogenetic analysis showed Shandong strains were distinct with prototype strains in every genotype. This report presents an overview of HEV-A in Shandong Province.
