Genetype characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008.
- Author:
Ya-Qing HE
1
;
Rui QI
;
Hong YANG
;
Hui-Xia XIAN
;
Xiang-Jie YAO
;
Fan YANG
;
Hai-Long ZHANG
;
Xiao-Ke YANG
;
Wen-Bo XU
Author Information
1. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. heyaqing1019@126.com
- Publication Type:Journal Article
- MeSH:
Cell Line;
China;
Enterovirus A, Human;
classification;
genetics;
isolation & purification;
Enterovirus Infections;
virology;
Feces;
virology;
Genotype;
Humans;
Molecular Sequence Data;
Phylogeny;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2009;25(6):415-419
- CountryChina
- Language:Chinese
-
Abstract:
Genetic characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008 were analyzed. All samples were detected by RT-PCR using EV71-specific primers. The VP1s of EV71 strains were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with those of subgenotype A, B and C using DNASTAR, BioEdit and Mega 3.1 softwares. The VP1 nucleotide sequences of 17 strains of Shenzhen shared 95.3%-99.4% identities with cluster C4a, and one strain shared 93.0%-95.6% identities with cluster C4b. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was 81.8%-86.0%. Among 18 strains, the homogeneity of the VP1 nucleotide sequence was between 92.5%-100%. All EV71 strains circulating in Shenzhen were of subgenotype C4. The predominant strain of EV71 belonged to cluster C4a, also there was cluster C4b.
