Subtype and sequence analysis of gag genes among HIV-1 strains circulating in Beijing during 2007.
- Author:
Jing-Rong YE
1
;
Yang LI
;
Li-Shi BAI
Author Information
1. Beijing Center for Disease Prevention and Control, Beijing 100013, China. basketball197602@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
China;
epidemiology;
Genotype;
HIV Infections;
epidemiology;
virology;
HIV-1;
classification;
genetics;
isolation & purification;
Humans;
Male;
Middle Aged;
Molecular Epidemiology;
Molecular Sequence Data;
Phylogeny;
Sequence Analysis, DNA;
Young Adult;
gag Gene Products, Human Immunodeficiency Virus;
genetics
- From:
Chinese Journal of Virology
2009;25(6):420-423
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Beijing during 2007 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routes, we collected the anti-conglutinated whole blood samples from HIV-1 newly infected individuals in Beijing during 2007 and separated plasma specimens from the aamples. RNAs were extracted and the gag genes from the various samples were amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analyses of gag genes were performed using the MEGA2 software. Among 200 HIV-1 plasma samples,161 gag HIV-1 gene fragments were amplified and analyzed. Seven HIV-1 subtypes or circulating recombinant forms (CRFs) of HIV-1 including A1 (1 strains), B (35 strains), Thai B (19 strains), C (3 strains), CRF01_AE (49 strains), CRF07_BC (51 strains), CRF08_BC (3 strain) were identified circulating in Beijing. The gene divergences inside the subtypes were different, with 7.7%, 6.5%, 6.7%, 6.7%, 5.5%, 4.3%, 5.8%, in subtype A1, B, Thai B, C, CRF01_AE, CRF07_BC, CRF08_BC, respectively. Subtypes CRF07_BC and CRF01_AE were predominant in Beijing account for 31.7% and 30.4% among samples. Seven HIV-1 subtypes exist in Beijing and the surveillance of HIV-1 gene variation should be paid more attention.
