Isolation and identification of avian leukosis virus-B from layer chickens infected with avian leukosis virus-J.
- Author:
Gong-Zhen LIU
1
;
Hong-Hai ZHANG
;
Qing LIU
;
Bo QIU
;
Feng WANG
;
Xiao-Wei WANG
;
Hong-Bo CHEN
;
Zi-Qiang CHENG
Author Information
1. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. gongzhenliu@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Avian Leukosis;
pathology;
virology;
Avian Leukosis Virus;
classification;
genetics;
isolation & purification;
Cell Line;
Chickens;
China;
Liver;
pathology;
virology;
Molecular Sequence Data;
Phylogeny;
Poultry Diseases;
pathology;
virology;
Spleen;
pathology;
virology
- From:
Chinese Journal of Virology
2009;25(6):445-451
- CountryChina
- Language:Chinese
-
Abstract:
Two strains of Avian leukosis virus subgroup B (ALV-B) were isolated for the first time in China Hy-line White on the cultured DF-1 cells which were inoculated tissue samples from by an ELISA assay, a histopathology examination and a PCR-based diagnosis. The results from the ELISA assay indicated that the positive rate of serum antibodies to ALV-B and ALV-J virus were 16.3% (15/92) and 13% (12/92), respectively. The histopathological examination indicated that two types of tumor cells existed at same focus in liver and spleen, which mainly were myelocytoma cells and lymphosarcoma cells. The PCR-based diagnosis were performed as follows: the cellular DNA was extracted from the inoculated DF-1 cells; the specific fragments of 1100 bp and 924 bp were obtained by a PCR system with the diagnostic primers of ALV-B and ALV-J; and the PCR results for ALV-A, MDV and REV were all negative. Then, the amplified fragments of the two ALV-B stains were partially sequenced and shown an identity of 92.8%,94.7% with the prototype strain of ALV-B (RSV Schmidt-ruppin B). The identities of two ALV-J strains with the prototype strain HPRS-103 at 96.9%, 91.5%; The identities of two ALV-J strains with the American prototype strain at 85.9%, 81.5%. Our study had shown that ALV-B was isolated for the first time from the ALV-J infected commercial layer flocks in China. It also indicated that the chance of genetic recombination among various subgroups of ALV was increased.
