Identification and genetic characterization of human enterovirus type 73, 75, and 97 strains of specie B isolated in Shandong province.
- Author:
Ze-Xin TAO
1
;
Hai-Yan WANG
;
Ai-Qiang XU
;
Li-Zhi SONG
;
Yan LI
;
Gui-Fang LIU
;
Yao LIU
;
Qing-Ying FAN
Author Information
1. Shandong Center for Disease Control and Prevention, Jinan 250014, China. zexin.tao@163.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Cell Line;
China;
Enterovirus;
classification;
genetics;
isolation & purification;
Enterovirus Infections;
virology;
Humans;
Molecular Sequence Data;
Paralysis;
virology;
Phylogeny;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2010;26(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
Molecular typing was conducted for three human enteroviruses (HEV) isolated from acute flaccid paralysis (AFP) cases in Shandong province, China. RNAs from virus supernatants were extracted and complete VP1 genes were amplified by RT-PCR and sequenced. Genotypes of these isolates were identified as HEV type 73, 75 and 97, respectively by BLAST program. Homology and phylogenetic tree analyses were performed. Sequence analysis of VP1 gene showed significant variation compared with prototype strains. This study presents the genetic characteristics of HEV 73, 75 and 97 of specie B in Shandong Province, and the first report of HEV97 in China.
