Establishment of a method for rapid detection of the nucleic acid of the novel A (H1N1) influenza virus.
- Author:
Da-Yan WANG
1
;
Rong-Bao GAO
;
Xiao-Dan LI
;
Wei WANG
;
Le-Ying WEN
;
Shu-Mei ZOU
;
Xiang ZHAO
;
Xi-Yan LI
;
Yu LAN
;
Lei YANG
;
Jun-Feng GUO
;
Zi LI
;
Yan-Hui CHENG
;
Min-Jju TAN
;
Xin-Wan LI
;
Yu-Hong ZENG
;
Yuan-Ji GUO
;
De-Xin LI
;
Yue-Long SHU
Author Information
1. China CDC, Institute for Viral Disease Control and Prevention, Key laboratory for Virus Gene Engineer, Beijing 100052, China. dayanwang@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Humans;
Influenza A Virus, H1N1 Subtype;
genetics;
isolation & purification;
Influenza, Human;
virology;
RNA, Viral;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
methods
- From:
Chinese Journal of Virology
2009;25 Suppl():1-3
- CountryChina
- Language:Chinese
-
Abstract:
A new flu caused by a novel influenza A(H1N1) virus has spread over the United States, Mexico and more than 40 other countries. And because of the immediate global concern, WHO has announced that the current level of influenza pandemic alert is raised to phase 5, indicating approaching of an influenza pandemic. As patients suffering from the influenza A (H1N1) have the similar symptoms as patients with seasonal influenza, differential detection and identification of the influenza virus have to depend on specific laboratory tests. We have successfully developed a RT-PCR based method for detection of the influenza A (H1N1) virus, and had applied the method to detection of clinical samples.
