Simultaneous detection of measles and rubella virus by multiplex real-time RT-PCR with an internal control.
- Author:
Bei-Bei YU
1
;
Yan FENG
;
Chang-Ping XU
;
Yi-Yu LU
;
Jing QIAN
Author Information
1. Department of Medical Microbiology and Parasitology, Medical school, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
Fluorescent Dyes;
Humans;
Measles;
diagnosis;
virology;
Measles virus;
genetics;
RNA, Viral;
genetics;
isolation & purification;
Reproducibility of Results;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Ribonuclease P;
genetics;
Rubella;
diagnosis;
virology;
Rubella virus;
genetics;
Sensitivity and Specificity
- From:
Chinese Journal of Virology
2010;26(2):109-114
- CountryChina
- Language:Chinese
-
Abstract:
Measles and rubella virus cause fever rash diseases that are uneasy to differentiate clinically from each other. Specific primers and fluorescence-labeled probes were designed, and a multiplex Real-time RT-PCR with an internal control was developed to simultaneously identify the measles and rubella virus. The multiplex Real-time RT-PCR assay was specific and no false positive or false negative results were found. The sensitivity of the assay was 0.1TCID50/mL and 1TCID50/mL for measles and rubella virus respectively. Analysis with 0.1-10(3)TCID50/mL measles or rubella virus samples demonstrated high validity and reproducibility with the coefficient of variability(CV) of below 0.9% for both measles and rubella virus. Using ribonuclease P (RNase P) as internal false negative control, the developed multiplex Real-time RT-PCR assay is suitable for rapid clinical diagnosis of measles and rubella virus.
