Molecular analysis of an avian influenza virus isolate of H5N2 subtype from parrot.
- Author:
Fen SHAN
1
;
Liang-meng WEI
;
Yan-mei WEI
;
Cheng-gang XU
;
Kai-jian LUO
;
Tao REN
;
Chao-an XIN
;
Pei-rong JIAO
;
Ming LIAO
Author Information
1. Key Lab of Animal Disease Control and Prevention of Ministry of Agriculture, Key Lab of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. shanfen83@163.com
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Evolution, Molecular;
Genes, Viral;
genetics;
Influenza A Virus, H5N2 Subtype;
genetics;
isolation & purification;
Influenza in Birds;
virology;
Parrots;
virology;
Phylogeny;
Sequence Analysis, DNA;
Viral Proteins;
chemistry;
genetics
- From:
Chinese Journal of Virology
2010;26(3):183-188
- CountryChina
- Language:Chinese
-
Abstract:
In 2005, an avian influenza virus stain was isolated from Parrot in Guangdong, which was then genotyped as H5N2 subtype and designated as A/Parrot/Guangdong/268/2005. According to the current OIE definition on the low-pathogenicity of avian influenza virus, the strain was recognized as a low pathogenic avian influenza virus due to the presence of one basic amino acid residue at the HA cleavage site. Some molecular characteristics of the virus, such as potential glycosylation sites in HA and NA, receptor binding sites of HA, and drug resistance site of NA, showed no variations. To analyze molecular evolution of this strain, we selected the sequences of H5N2 subtype AIVs from GenBank and established the phylogenetic trees. Our results indicated that this strain shared the highest homologies with the H5N2 LPAI isolate A/Pheasant/NJ/1355/1998-like. Phylogenic analysis revealed the isolate, together with A/Chicken/Pennsylvania/1/1983 (H5N2), belonged to America lineages and clustered with A/Pheasant/NJ/1355/1998-like.
