Construction, expression and immunogenicity analysis of a fusion protein containing M2e of influenza A virus fused to a modified Pseudomonas aeruginosa exotoxin A.
- Author:
Yi XU
1
;
Li-hong YAO
;
Ai-jun CHEN
;
Jian-qiang GUO
;
Xiao-yu LIU
;
Hong BO
;
Li-qi LIU
;
Yue-long SHU
;
Zhi-qing ZHANG
Author Information
1. National Key Laboratory of Molecular Virology and Genetic Engineering, Institute of Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
- Publication Type:Journal Article
- MeSH:
ADP Ribose Transferases;
genetics;
Animals;
Bacterial Toxins;
genetics;
Enzyme-Linked Immunosorbent Assay;
Escherichia coli;
genetics;
Exotoxins;
genetics;
Female;
Gene Expression;
Immunization;
Influenza A virus;
immunology;
physiology;
Lung;
immunology;
virology;
Mice;
Mice, Inbred BALB C;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
immunology;
isolation & purification;
Viral Matrix Proteins;
biosynthesis;
genetics;
immunology;
isolation & purification;
Virulence Factors;
genetics
- From:
Chinese Journal of Virology
2010;26(3):189-194
- CountryChina
- Language:Chinese
-
Abstract:
M2 protein of type A influenza virus is a good candidate for universal influenza vaccine, exotoxin A of Pseudomonas aeruginosa may facilitate the immunogenicity of M2 protein. We constructed and expressed a prokaryotic expression plasmid containing a chimeric gene of M2 extracellular coding region and a partial PEA gene, and observed the immunoprotection in BALB/c mice vaccinated with the fusion protein. The fusion protein (ntPE-M2e) was generated by inserting the coding sequence of the M2e in place of Ib loop in PEA. This fusion protein was used to immunize BALB/c mice by subcutaneously injection with incomplete Freund's adjuvant and boost at weeks 3 and 7. The immunized mice were challenged with influenza virus strain A/PR/34/8. The fusion protein (ntPE-M2e) immunization protected mice against lethal viral challenge. ELISA and ELISPOT results demonstrated that the fusion protein could induce a strong systemic immune response against synthetic M2e peptide, and virus replication in the lungs of mice was inhibited in comparison with the control. This study provides foundation for developing broad-spectrum vaccines against type A influenza viruses.
