Genome sequencing and analysis of the bovine viral diarrhea virus-2 strain JZ05-1 isolated in China.
- Author:
Qing-chao LI
1
;
Li-guang MIAO
;
Hai-tao LI
;
Yan-huan LIU
;
Guang-lei ZHANG
;
Jia-mei XIAO
Author Information
1. Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109, China. Sman9@126.com
- Publication Type:Journal Article
- MeSH:
5' Untranslated Regions;
genetics;
Animals;
Cattle;
China;
Diarrhea Virus 2, Bovine Viral;
classification;
genetics;
isolation & purification;
Genome, Viral;
genetics;
Genotype;
Molecular Sequence Data;
Phylogeny;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:
Chinese Journal of Virology
2010;26(3):238-243
- CountryChina
- Language:Chinese
-
Abstract:
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, which is a widespread problem for beef and dairy herds, and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin, China generated cytopathic effect (CPE) in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced, the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results, the JZ05-1 genome was composed of 12285 nucleotides in length (GenBank accession No. GQ888686), which could be divided into three regions: a 387 nt 5'-untranslated region (UTR), a 11694 nt single large open reading frame encoding a polyprotein, and a 204 nt 3'-UTR. The 5'-UTR and genome sequences were analyzed by sequence alignment and construction of phylogenetic trees. The strain JZ05-1 was classified as BVDV type 2a. The BVDV-2 strain JZ05-1 genome showed high similarity to the p11Q isolated in Canada and the XJ-04 isolated in China, with 90% and 91% identity in nucleotide sequence, respectively. Compared with the similarity within the BVDV-2 genotype (96%), the JZ05-1 had low sequence similarity to other BVDV-2 strains.
