Complete genome sequence of Coxsackievirus B5 (CVB5/09) strain isolated in China and its phylogenetic analysis.
- Author:
Yong-Feng HU
1
;
Li-Na ZHAO
;
Jie DONG
;
Zhi-Qiang WU
;
Jiang DU
;
Ying XUE
;
Fan YANG
Author Information
1. State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. huyf15@sina.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
China;
Coxsackievirus Infections;
virology;
Enterovirus B, Human;
classification;
genetics;
isolation & purification;
Genome, Viral;
Humans;
Molecular Sequence Data;
Phylogeny;
Viral Proteins;
genetics
- From:Chinese Journal of Virology
2010;26(4):283-289
- CountryChina
- Language:Chinese
-
Abstract:
An outbreak of hand foot and mouth disease occurred in Shang dong, China in 2009. Almost 20% of patient's swabs was positive for Coxsackie virus B5 (CVB5) identified by RT-PCR and sequencing. It was suggested that CVB5 may be another important pathogen for HFMD. Fifteen pairs of overlapping primers were designed and the genome sequence was sequenced. The genome of CVB5 was 7 399 nt in length, coding for 2 185aa. The genome displayed 80.6%-85.3% nucleotide sequence identity and 96.1%-96.9% amino acid sequence identity with another three CVB5 respectively. Phylogenetic analysis showed that different segment of genome underwent a distinct evolutionary and selective pressure. Simplot analysis displayed no evident recombination between genome of CVB5 and other HEV B viruses. The complete and characterized genome of CVB5/09 provides further insight into the genetics of CVB5 and other HEV B viruses, aiding in the surveillance and control of HFMD.
