Genome sequencing and analysis of a peste des petits ruminants virus isolate, China/Tib/07.
- Author:
Wen-Hua LIU
1
;
Jing-Yue BAO
;
Xiao-Dong WU
;
Zhi-Liang WANG
Author Information
1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Cercopithecus aethiops;
China;
Genome, Viral;
Molecular Sequence Data;
Peste-des-Petits-Ruminants;
veterinary;
virology;
Peste-des-petits-ruminants virus;
classification;
genetics;
isolation & purification;
Phylogeny;
Sequence Alignment;
Sequence Analysis, DNA;
Sheep;
Sheep Diseases;
virology;
Vero Cells;
Viral Proteins;
genetics
- From:
Chinese Journal of Virology
2010;26(4):322-329
- CountryChina
- Language:Chinese
-
Abstract:
Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae. The complete genome of a Peste des petits ruminants virus (PPRV) isolate, China/Tib/07 was sequenced and molecular characteristics was analyzed. The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank, while the sequences of the 3' and 5' ends of the genome were determined by RACE. Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0. Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length, encoding six structural proteins and two non-structural proteins just like other known PPRV genomes. Phylogenetically, the virus genome shared homology of 91.6%-98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.
