Bombesin increases cytokeratin expression and free calcium concentration in PC-3 cell line.
- Author:
Bin WU
1
;
He WANG
;
Wei-jun QING
Author Information
- Publication Type:Journal Article
- MeSH: Bombesin; pharmacology; Calcium; analysis; Cytoskeleton; drug effects; metabolism; Fluoroimmunoassay; Humans; Keratins; biosynthesis; Male; Microscopy, Confocal; Prostatic Neoplasms; metabolism; Serum Albumin, Bovine; Tumor Cells, Cultured
- From: National Journal of Andrology 2007;13(4):332-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of bombesin noncytoskeleton form and intracellular free calcium ([Ca2+]i) concentration in PC-3 prostate cancer cell line.
METHODSImmunofluorescent histochemistry (IH) combined with laser scanning confocal microscopy (LSCM) was used to examine the expression of cytokeratin (CK) in PC-3 cells treated with definite concentrations of BBS and observe its effect on cytoskeleton form. Fluo-3/AM fluorescence technique and LSCM were adopted to measure the [Ca2+]i concentration after different concentrations (10(-9), 10(-7) and 10(-5) mol/L) of BBS were added in PC-3 cells.
RESULTSBBS (10(-5) mol/L) stimulated the expression of CK in PC-3 cells and the formation of lamellipodium, and increased the [Ca2+]i concentration, with concentration dependence.
CONCLUSIONDefinite concentrations of BBS could obviously enhance the [Ca2+] i concentration, CK expression and cytoskeleton morphology of PC-3 cells. The results provide a basis for further studies on the role of BBS in tumour researches as well as in intracellular signal transmission.
