Morphological and proinflammatory expression of the experimental autoimmune prostatitis in the rat model.
- Author:
Lei CHEN
1
;
Wei-ping XIA
;
Zhi-heng ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autoimmune Diseases; pathology; Chemotactic Factors, Eosinophil; genetics; Disease Models, Animal; Gene Expression; Interleukin-4; blood; Male; Polymerase Chain Reaction; Prostatitis; pathology; Random Allocation; Rats; Rats, Wistar
- From: National Journal of Andrology 2007;13(5):444-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a Wistar rat model of chronic abacterial prostatitis (CAP) by injecting purified prostate protein and Freund's complete adjuvant, and to study the influence on the morphology and proinflammatory expression.
METHODSMale rats were injected with the Pertussis-Diphteria-Tetanus vaccine into the abdominal cavity and purified prostate protein and Freund's complete adjuvant intradermally at 0 and 30 days. At 60 days, the rats were sacrificed, and then the prostate specimens were observed, under the light microscope and electron microscope, and the changes of proinflammatory expression was observed too, using PCR technique.
RESULTSThe products of proinflammatory expression, such as eotaxin, iNOS and IL-4 increased markedly. The change of chronic inflammation was shown by light microscope and electron microscope.
CONCLUSIONChronic prostatitis is associated with autoimmunity.
