Voltage-dependent anion channels (VDAC) on human sperm membrane.
- Author:
Zeng-jun WANG
1
;
Wei ZHANG
;
Hong-fei WU
;
Yuan-geng SUI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Gene Expression; Humans; Male; Polymerase Chain Reaction; Recombinant Proteins; biosynthesis; Signal Transduction; physiology; Spermatozoa; metabolism; Testis; metabolism; Voltage-Dependent Anion Channels; biosynthesis; physiology
- From: National Journal of Andrology 2007;13(6):498-501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.
METHODSVDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.
RESULTSThe gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.
CONCLUSIONVDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
