Detection of transcriptional activities of tumor-specific survivin promoter in human prostatic carcinoma.
- Author:
Xiao-mei LUO
1
;
Jia-yun LIU
;
Ming-quan SU
;
Xiao-ke HAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Inhibitor of Apoptosis Proteins; Male; Microtubule-Associated Proteins; biosynthesis; genetics; Neoplasm Proteins; biosynthesis; genetics; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Prostatic Neoplasms; genetics; metabolism; Transfection
- From: National Journal of Andrology 2007;13(6):502-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone DNA sequence of the survivin promoter and study is transcriptional activities in human prostate cancer cells and normal Chang liver cells.
METHODSThe fragment of the survivin promoter was acquired by PCR amplification and inserted into pPRIME vectors to reconstruct a recombinant plasmid named pPRIME-S1pro and pPRIME-S2pro. Then the reconstructed plasmid was transiently transfected into human prostate cancer cells lines LNCaP and normal Chang liver cells. The transcriptional activities of the survivin promoter in various cells was determined by measuring the expression of green fluorescent protein (GFP).
RESULTSThe survivin promoter had transcriptional activities in LNCaP cells and the transcriptional activity of the S2pro was much higher that of the S1pro, reaching a level of 39% of the transcriptional activity of the CMV promoter.
CONCLUSIONThe survivin promoter cloned in the therapy for prostate cancer.
