Comparative analysis of proteins in seminal plasma of non-obstructive azoospermia patients and healthy fertile males.
- Author:
Jie BAI
1
;
Ling SUN
;
Shi-Ling CHEN
;
Li-Wen ZHANG
;
Jun-Long MA
;
Yu-Long CONG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Azoospermia; metabolism; Humans; Male; Proteins; analysis; Proteomics; methods; Semen; chemistry; cytology; Spectrometry, Mass, Electrospray Ionization; Sperm Count; Sperm Motility
- From: National Journal of Andrology 2007;13(7):579-583
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyse protein alterations in the seminal plasma of non-obstructive azoospermia patients.
METHODSSemen samples were collected from 11 healthy fertile and 6 azoospermia male volunteers respectively and tested by SELDI-TOF-MS with CM10 protein chip to get protein spectra maps, which were automatically treated with the special softwares of Ciphergen Inc.
RESULTSThe mean peak heights of 28 proteins expressed in the seminal plasma of the azoospermia patients were statistically different from those of the healthy fertile males (P < 0.05 ), of which 24 were of lower contents than in the normal controls, 4 with remarkably significant difference, M/Z 7 196.058, 7 630.573, 7 547.610 and 7 709.833 (P < 0.01).
CONCLUSIONThe seminal plasma proteins of the azoospermia patients were significantly different from those of the healthy fertile males, with decreased contents of most of the different proteins, which might be significantly correlated with the development of azoospermia.
