Expression of phosphorylated ERK1/2 induced by crocidolite fibers in BEAS-2B cells.
- Author:
Xin-chao WANG
1
;
Yi-ming WU
;
James M SAMET
;
Adrew J GHIO
Author Information
- Publication Type:Journal Article
- MeSH: Asbestos, Crocidolite; toxicity; Bronchi; cytology; Cells, Cultured; Epidermal Growth Factor; pharmacology; Epithelial Cells; drug effects; metabolism; Humans; MAP Kinase Kinase 1; metabolism; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase 3; metabolism; Phosphorylation
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):597-600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the characteristic of the signal transduction in BEAS cells induced by the crocidolite fibers.
METHODSThe human respiratory airway epithelial cells BEAS-2B were cultured in vitro. The final 100 microg/ml crocidolite concentration and lOnM of epidermal growth factor were cocultured with BEAS-2B cells for 30 minutes and 120 minutes. Phosphorylated ERKl/2 and MEKl/2 were detected by Western Blotting using specific antibodies.
RESULTSA rapid phosphorylation expression of ERK1/2 (molecular weight at 44 kD and 42 kD, also called as p44 and p42) was observed by treatment of the BEAS-2B cells with 100 microg/ml crocidolite or 100 ng/ml EGF (the proven activator of the ERK signaling pathway) at 30 minutes. This phosphorylation could be still detected by incubation the cells at 2 hours. However no expression was changed for the total ERKl/2 expression at 30 minutes or 120 minutes. Treatment of BEAS cells with 100 microg/ml crocidolite fiber or 100 ng/ml EGF led to the rapid increased phosphorylation of MEK1/2 at 30 minutes; similarly, the overexpression of MEK1/2 could last 2 hours.
CONCLUSIONThe crocidolite induces the MAPK (ERK1/2 and MEK1/2) phosphorylation within a shorter time. It indicates that the MAPKs signals are involved in the process of crocidolite induced damage.
