Effects of sodium arsenite on catalase in human keratinocytes.
- Author:
Xian-ce SUN
1
;
Feng-yuan PIAO
;
Yi WANG
;
Yuan-yuan XU
;
Xin LI
;
Bing LI
;
Ya-ping JIN
;
Gui-fan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Arsenites; toxicity; Blotting, Western; Catalase; biosynthesis; genetics; Cell Line; Dose-Response Relationship, Drug; Humans; Keratinocytes; drug effects; enzymology; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sodium Compounds; toxicity
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(10):605-607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).
METHODSThe activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.
RESULTSIf the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).
CONCLUSIONCAT is inhibited by sodium arsenite in the transcription, translation and activity levels.
