Scanning aneugen and clastogen by micronuclei analysis using flow cytometry.
- Author:
Ming-Jie YANG
1
;
Jian-Chang ZHOU
;
Zhi LI
;
Xing-Fen YANG
;
Jun-Ming HUANG
;
Xiao-Hua TAN
;
Jia CAO
;
Rui-Ping ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Colchicine; toxicity; Cyclophosphamide; toxicity; Flow Cytometry; methods; Male; Mice; Micronuclei, Chromosome-Defective; Mutagens; toxicity; Reticulocytes; drug effects; ultrastructure
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(11):649-652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.
METHODSCells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.
RESULTSThe ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.
CONCLUSIONThe flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
