Differential proteomic expression in human liver cells stimulated by hydroquinone.
- Author:
Li JU
1
;
Shu-Zhi ZHANG
;
Ran ZHAO
;
Geng-Dong YAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Electrophoresis, Gel, Two-Dimensional; Hepatocytes; drug effects; metabolism; Humans; Hydroquinones; toxicity; Mass Spectrometry; Proteomics; Reproducibility of Results
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(11):658-661
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the differential proteomic expression in human liver cells L-02 after exposure to HQ.
METHODSSubcultured L-02 cells were treated by HQ for 24 h at a 1 x 10(-4) mol/L concentration and a blank group was set as the control. Immediately after the treatment, total cellular proteins were extracted and separated by 2-DE, and the images were analyzed by PDQuest software. The experiment was totally repeated 3 times with 3 repetitions for each group every time. The well repeated spots were identified by MALDI-TOF MS and then searched in NCBI human protein database with Mascot.
RESULTSAbout 1,000 spots per gel were found. Compared with the control group, 17, 18 and 24 spots were significantly altered in 3 separate experiments. The 4 well repeated spots were identified by MALDI-TOF MS as Rho GDP dissociation inhibitor GDI alpha, 6-phosphogluconolactonase, erbB3 binding protein EBP1 and lamin A/C, isoform 1 precursor. They were involved in cell skeleton, signal transduction and energy metabolization in functional classification.
CONCLUSIONHydroquinone can change the protein expression in liver cells, which provides clues for exploring the toxic mechanism.
