Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells.
- Author:
Jing DENG
1
;
Guo-Yuan CHEN
;
Jia-Jia JI
;
Hao TAN
;
Song WANG
;
Feng-Xian HU
;
Lian-Hua CHAI
;
Si-Hai LIU
;
Wei-Dong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Carbon Disulfide; toxicity; Cell Line; Cell Survival; Fas Ligand Protein; metabolism; Male; Rats; Sertoli Cells; drug effects; metabolism; Testis; cytology
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(12):730-733
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.
METHODSSertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.
RESULTSSertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).
CONCLUSIONCS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
