Changes of apoptosis, mitochondrion membrane potential and Ca2+ of hypothalamic neurons induced by high power microwave.
- Author:
Li MENG
1
;
Rui-Yun PENG
;
Ya-Bing GAO
;
Shui-Ming WANG
;
Jun-Jie MA
;
Wen-Hua HU
;
De-Wen WANG
;
Zhen-Tao SU
;
Bo DONG
;
Tian-Hao XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; radiation effects; Calcium; metabolism; Cells, Cultured; Hypothalamus; cytology; radiation effects; Membrane Potential, Mitochondrial; radiation effects; Membrane Potentials; Microwaves; adverse effects; Neurons; metabolism; radiation effects; Rats; Rats, Wistar
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(12):739-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure.
METHODSPrimarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons.
RESULTSThe ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure.
CONCLUSIONApoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.
