Role of uteroglobin-binding protein in antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.
- Author:
Tian-Jie ZHANG
1
,
2
,
3
,
4
;
ivyandl.li@hotmail.com.
;
Jian-Zhong HAN
;
Hui-Jun LIU
;
Xiao-Hong LIAO
;
Chen LI
;
Zi-Qiang LUO
Author Information
1. Department of Physiology, Central South University, Changsha 410078, China
2. Department of Physiology, Xiangnan College, Chenzhou 423000, China
3. Department of Physiology, Changzhi Medical College, Changzhi 046000, China. E-mail: luozq1962@163.com
4. ivyandl.li@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Gene Expression;
Interleukin-10;
metabolism;
Lipopolysaccharides;
Macrophages;
metabolism;
Mice;
Peptide Fragments;
metabolism;
RNA, Messenger;
Uteroglobin;
metabolism
- From:
Acta Physiologica Sinica
2013;65(4):363-369
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secretion from macrophages, and this effect is mediated by UGBP.
