Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca(2+)-sensing receptor-evoked extracellular Ca(2+) influx and NO generation in human umbilical vein endothelial cells.
- Author:
Hui ZHAO
1
,
2
;
Xiao LIANG
;
Hua ZHONG
;
Chun-Jun ZHANG
;
Fang HE
Author Information
1. Department of Pathophysiology/Key Laboratory of Education Ministry of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi 832002, China
2. Department of Lymphoma and Breast Medicine, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China. fangf2002shz@126.com.
- Publication Type:Journal Article
- MeSH:
Calcium;
physiology;
Calcium Channel Blockers;
pharmacology;
Calcium Channels;
physiology;
Calcium Signaling;
Fluoresceins;
pharmacology;
Human Umbilical Vein Endothelial Cells;
physiology;
Humans;
Nitric Oxide;
biosynthesis;
Nitric Oxide Synthase Type III;
metabolism;
Receptors, Calcium-Sensing;
physiology
- From:
Acta Physiologica Sinica
2013;65(5):553-561
- CountryChina
- Language:Chinese
-
Abstract:
This paper aims to investigate the effect of store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC) on Ca(2+)-sensing receptor (CaR)-induced extracellular Ca(2+) influx and nitric oxide (NO) generation in human umbilical vein endothelial cells (HUVEC). SOC blocker, non-selective cation channel blocker, ROC agonist and ROC blocker were used separately and combined. Intracellular Ca(2+) concentration ([Ca(2+)]i) was measured by Fura-2/AM loading. The activity of endothelial nitric oxide synthase (eNOS) and the production of NO were determined by the DAF-FM diacetate (DAF-FM DA). The results showed that increases of [Ca(2+)]i, eNOS activity and NO generation induced by CaR agonist Spermine were all reduced after single blocking the SOC or ROC, respectively (P < 0.05). ROC agonist can partially abolish the ROC blocker's effect (P < 0.05). The above mentioned effects evoked by CaR agonist Spermine were further reduced when blocking both SOC and ROC than single blocking SOC or ROC in HUVEC (P < 0.05). In conclusion, these results suggest that the SOC and ROC participate in the processes of CaR-evoked extracellular Ca(2+) influx and NO generation by a synergistic manner in HUVEC.
