Precursor brain-derived neurotrophic factor reduces survival and axonal sprouting of rat spiral ganglion neurons in vitro.
- Author:
Hua TONG
1
;
Lei ZHOU
;
Jian-Ping LIU
;
Li GAO
;
Na SHEN
;
Xin-Sheng HUANG
Author Information
1. Department of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai 200032, China. huangxinsh@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Axons;
physiology;
Brain-Derived Neurotrophic Factor;
pharmacology;
Cell Survival;
Cells, Cultured;
JNK Mitogen-Activated Protein Kinases;
antagonists & inhibitors;
MAP Kinase Signaling System;
Neurites;
physiology;
Neurons;
cytology;
Protein Precursors;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Spiral Ganglion;
cytology
- From:
Acta Physiologica Sinica
2014;66(2):151-157
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the effect of precursor brain-derived neurotrophic factor (proBDNF) on survival and neurite outgrowth of cultured rat spiral ganglion neurons (SGNs). Spiral ganglions (SG) were collected from postnatal day 5 Sprague Dawley (SD) rats, then enzymatically digested and suspended. Dissociated SGNs were plated on poly-D-lysine/laminin coated eight-well chamber plates and maintained at 37 °C for 4 h to promote the attachment of the neurons. Cultured SGNs were randomly divided into five groups: control group, BDNF group (BDNF 10 ng/mL), C10 group (proBDNF 10 ng/mL), C50 group (proBDNF 50 ng/mL), and C100 group (proBDNF 100 ng/mL). All groups were incubated in a serum-free medium. 48 h after incubation, SGNs were fixed and stained for βIII tubulin. Immunostaining of the cultured SGNs showed that, compared with the control group, the cellular survival of C50 group and C100 group were significantly reduced (P < 0.001). Furthermore, surviving numbers of the three proBDNF-treated groups were all lower than the BDNF group. In order to assess the effect of proBDNF on cell morphology, SGNs were divided into two categories: SGNs with or without neurites. The results demonstrated that proBDNF significantly increased the proportions of SGNs without neurites in C10, C50 and C100 groups compared with that in control group (P < 0.001). In addition, c-Jun N-terminal kinase (JNK) inhibitor, SP600125 (20 μmol/L) significantly increased the surviving number of SGNs in C50 group. These results suggest that proBDNF reduces the survival rate of cultured SGNs and inhibits the sprouting of neurites. Furthermore, the inhibition of JNK signaling attenuates the effect of proBDNF on SGNs survival.
